Media and Buffers in Dissolution Method Development

Sink Conditions: Volume of medium should be at least 3 times that needed to form a saturated solution (USP definition).

pH Range: Typical range is 1.1 to 6.8, but can be adjusted up to pH 8.0 for solubility.

Common Buffers:

  • Hydrochloric acid: pH 1.0–3.0
  • Glycine: pH 2.0–3.0
  • Citrate: pH 2.5–3.5
  • Acetate: pH 4.0–5.5
  • Phosphate: pH 6.0–8.0

Surfactants:

  • Use sodium dodecyl sulfate (SDS) as the first choice.
  • Typical SDS concentration: 0.1% to 3% (adjust if needed for acceptable dissolution).
  • If SDS doesn’t work, explore other FDA-approved surfactants.

Medium Deaeration: Ensure medium is deaerated to prevent bubbles, which can interfere with dissolution.

Medium Volume and Time Points

  • Standard volumes: 500 mL and 900 mL are commonly used and accepted by regulatory agencies.
  • Typical Sampling Times: 15, 30, 45, 60 minutes.
  • Early Time Points (e.g., 5–10 minutes): If monitoring disintegration or rapid dissolution.
  • Extended Time Points: If concerned about stability or slower dissolution.
  • 15-min Sampling: Often useful for compounds that dissolve ≥85% in this time.

Sinkers

  • Use of Sinkers: For capsules or sticky tablets to prevent adherence to the vessel and ensure consistent dissolution.
  • Helps avoid variability caused by non-centered tablets or dosage forms.


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Resource Person: Muhammad Taaha Khan

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